Homobifunctional Cross-Linkers
Two identical reactive ends
Bis(β-[4-azidosalicylamido]-ethyl) disulfide
Iodinatable
Features
Molecular Weight: 474.52
Spacer Arm (Å): 21.3
Reactive Toward: Non Selective
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: Reducing Agents (Thiols)
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(Bis(2-[Succinimidooxycarbonyloxy]ethyl) sulfone
Features
Molecular Weight: 436.35
Spacer Arm (Å): 13
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: Base
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1,4-Di-(3’-[2’pyridyldithio]-propionamido) butane
Features
Molecular Weight: 428.71
Spacer Arm (Å): 19.9
Reactive Toward: Sulfhydryls
Membrane Permeable: not done
Water Soluble: NO
Cleavable/ Reversible: Reducing Agents (Thiols)
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Dithiobis(succinimidyl propionate)
Features
Molecular Weight: 404.42
Spacer Arm (Å): 12
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: Reducing Agents (Thiols)
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Disuccinimidyl suberate
Ideal for receptor ligand cross-linking and coupling antibody to Protein A or G for immunoprecipitations.
OneQuant™ DSS is supplied as 8 vials of 2mg DSS, suitable for preparing for ~100µl 50mM stock solution from each vial.
Features
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Disuccinimidyl tartrate
Features
Molecular Weight: 344.24
Spacer Arm (Å): 6.4
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: Oxidizing Agents (Periodate)
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3,3’-Dithiobis(sulfosuccinimidyl propionate)
Features
Molecular Weight: 608.51
Spacer Arm (Å): 12
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: Reducing Agents (Thiols)
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Ethylene glycol bis(succinimidyl succinate)
Ideal for receptor ligand crosslinking
Features
Molecular Weight: 456.36
Spacer Arm (Å): 16.1
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: Hydroxylamine
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Sulfodisuccinimidyl tartrate
Features
Molecular Weight: 548.32
Spacer Arm (Å): 6.4
Reactive Toward: Primary Amines
Membrane Permeable: NO
Water Soluble: YES
Cleavable/ Reversible: Oxidizing Agents (Periodate)
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