Synonyms: Thiol Reactive Agarose Beads, Activated thiol Sepharose
Thiopropyl Resin is used in purification of thiol group containing proteins, molecules and enzymes. The thiol-containing molecules are reversibly immobilized on the resin. When the sample to be purified is passed through the resin, thiol- containing biomolecules bind to the resin to form mixed disulfides and release2-thiopyridone. The bound molecule is eluted by addition of reducing agent such as DTT or 2-mercaptoethanol.
Specifications
- Active group: 2-pyridyl disulfide
- Active group concentration: 18-31 µmole 2-pyridyl disulfide/ml drained gel
- Spacer: 3-Carbon-SH group
- Coupling capacity: around 10-20 mg protein/ml of drained gel
- Matrix: 6% cross-linked Agarose
- pH stability: 2-8 long term, 2-8 for CIP
- Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Do not use azides.
- Physical stability: volume variations are negligible for changes in pH or ionic strength
- Supplied as 50% slurry in 20% ethanol, 1 mM EDTA
Applications
- Purify thiol-containing proteins and peptides from non-thiol containing proteins or peptides.
- Purify enzyme with active thiol site from denatured enzymes
- Store and protect thiol containing
